Lyfesate™

Lyfesate™ is a xeno-free, human-derived cell culture supplement developed as an alternative to fetal bovine serum for research use.

Human platelet lysate provides a more physiologically relevant culture environment for human cells, supporting reproducible expansion without relying on animal-derived serum.

Why Lyfesate?

Xeno-free and human-derived
Low batch-to-batch variability
Reliable and scalable supply
Closed-system production
Pathogen-inactivated source material
Full traceability and batch documentation
No heparin required

Research Applications

Lyfesate™ supports consistent expansion across established and emerging research workflows, including:

Cell therapy research
Regenerative medicine research
Translational research
Process development
Tissue engineering
Advanced therapeutic development

Supporting Consistent Cell Expansion

Broad compatibility across commonly used human cell types.

Mesenchymal stromal cells

T-cells

Dermal fibroblasts

Keratinocytes

Endothelial cells

Trusted European Experience

Lyfesate is produced by Lysantum, part of Rode Kruis-Vlaanderen (Belgian Red Cross Flanders), which brings more than a decade of experience producing and supplying high-quality human platelet lysate to the European research market.

Source material is collected from consenting, non-remunerated voluntary donors. The referenced Ultra batch contains material from 480 donors, with source products tested for HIV NAT, HBV NAT, and HCV NAT.

Product Availability

Lyfesate is offered in two research-use configurations to support different media requirements and stages of development.

PREMIUM

Optimized for reliable performance in well-established cell culture systems.

ULTRA

Designed for maximum versatility across cell types, media platforms, and emerging workflows.

Human Platelet Lysate (hPL)

Field	Information
Product	LYSANTUM PREMIUM/ULTRA
Intended use	Research use only
Reference number	P480R / P480R-0500 / P480T
Lot number	PL20260225
Expiration date	25/02/2028
Shipping conditions	Dry ice
Storage temperature	≤ -20°C
PREMIUM	Optimized for established cell cultures.
ULTRA	Maximum versatility across cell workflows.

Analysis of the Final Product

Test	Specification	Result
Sterility	No aerobic and anaerobic growth	✓
Endotoxin	< 5.00 EU/mL	✓
Mycoplasma	Below detection limit	✓
Total protein	40–80 g/L	✓
Ca²⁺	≤ 2.0 mM	✓
Osmolality	280–340 mOsm/kg	✓
pH	6.5–8.5	✓
Cell growth performance	Proliferation of human adipose-derived stem cells (hADSC)	✓